Data/hora: 03/06/2024 12:30:49

Provedor de dados: 	Brazilian Journal of Pharmaceutical Sciences	
País: 	Brazil	
Título: 	β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil	
Autores: 	Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.	
Resumo: 	ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic extended spectrum β-lactamase (ESBL) test was positive in 53.8% of the K. pneumoniae isolates, 100.0% of the E. coli isolates, and 100.0% of the E. aerogenes isolates. Phenotypic and genotypic testing of K. pneumoniae carbapenemase (KPC) was positive in 50.0% of the K. pneumoniae isolates. Phenotypic and genotypic testing showed that none of the P. aeruginosa or Acinetobacter spp. isolates were positive for metallo- β-lactamase (MBL). The bla OXA gene was detected only in Acinetobacter spp. The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp. isolates, indicating the inadequate dissemination control of multidrug-resistant bacteria in this hospital environment.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902017000216111	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.53 n.2 2017	
Fonte: 	info:eu-repo/semantics/openAccess